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fitc anti mouse f4 80  (Elabscience Biotechnology)


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    Elabscience Biotechnology fitc anti mouse f4 80
    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis <t>of</t> <t>F4/80</t> and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
    Fitc Anti Mouse F4 80, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+anti+mouse+f4+80/FITC+Anti-Mouse+F4%2F80+Antibody/pmc13091351-201-5-35
    Average 95 stars, based on 76 article reviews
    fitc anti mouse f4 80 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment"

    Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

    Journal: Molecular Therapy Oncology

    doi: 10.1016/j.omton.2026.201185

    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
    Figure Legend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Techniques Used: Flow Cytometry

    Related Articles

    Staining:

    Article Title: Rab11b promotes M1-like macrophage polarization by restraining autophagic degradation of NLRP3 in alcohol-associated liver disease.
    Article Snippet: Macrophage polarization is vital to mounting a host defense or repairing tissue in various liver diseases.. Excessive activation of the NLR family pyrin domain containing 3 (NLRP3) inflammasome is related to the orchestration of inflammation and alcohol-associated liver disease (ALD) pathology.. Rab GTPases play critical roles in regulating vesicular transport.

    Article Title: STING deficiency alleviates ischemia-reperfusion injury via JAK2/STAT3-mediated macrophage polarization and autophagy.
    Article Snippet: Intestinal ischemia–reperfusion (IIR) injury triggers severe inflammation and epithelial barrier damage, often leading to systemic complications.. The stimulator of interferon genes (STING), a key regulator of innate immunity, has been implicated in inflammatory responses, but its role in IIR remains unclear.. This study aimed to determine whether STING influences intestinal injury through macrophage polarization and autophagy regulation via the JAK2/STAT3 signaling pathway.

    Flow Cytometry:

    Article Title: STING deficiency alleviates ischemia-reperfusion injury via JAK2/STAT3-mediated macrophage polarization and autophagy.
    Article Snippet: Intestinal ischemia–reperfusion (IIR) injury triggers severe inflammation and epithelial barrier damage, often leading to systemic complications.. The stimulator of interferon genes (STING), a key regulator of innate immunity, has been implicated in inflammatory responses, but its role in IIR remains unclear.. This study aimed to determine whether STING influences intestinal injury through macrophage polarization and autophagy regulation via the JAK2/STAT3 signaling pathway.



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    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis <t>of</t> <t>F4/80</t> and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
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    f4 80  (Elabscience Biotechnology)
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    Experimental design for cutaneous wound model and treatment. (A) Scheme of R 2 F 4 R 2 for treating cutaneous wound; (B) Schematic images of wound contraction during 6 d; (C) SEM images of E. coli entrapped by R 2 F 4 R 2 in vivo . Scale bar: 5 µm and 2 µm (magnification); (D) Histopathological H&E staining of skin tissues on Day 3 and 6. The red arrow: epidermis. Blue arrows: ermis; black dashed line: the boundary of epidermis and dermis. Black arrow: the infiltration of inflammatory cells; red circle: hair follicles; black circle: the vasodilation and congestion. Scale bar: 100 µm and 50 µm (magnification); (E) Immunofluorescence analysis of KI67, M1 <t>(CD86-F4/80</t> and IL-6) and M2 (CD206-F4/80 and IL-10) macrophages markers. Scale bar: 100 µm; (F) MASSON staining of skin tissues on Day 3 and 6. Black square: collagen deposition; black dashed line: the boundary of epidermis and dermis; black circle: hair follicles. Scale bar: 200 µm and 100 µm (magnification).
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    Experimental design for cutaneous wound model and treatment. (A) Scheme of R 2 F 4 R 2 for treating cutaneous wound; (B) Schematic images of wound contraction during 6 d; (C) SEM images of E. coli entrapped by R 2 F 4 R 2 in vivo . Scale bar: 5 µm and 2 µm (magnification); (D) Histopathological H&E staining of skin tissues on Day 3 and 6. The red arrow: epidermis. Blue arrows: ermis; black dashed line: the boundary of epidermis and dermis. Black arrow: the infiltration of inflammatory cells; red circle: hair follicles; black circle: the vasodilation and congestion. Scale bar: 100 µm and 50 µm (magnification); (E) Immunofluorescence analysis of KI67, M1 <t>(CD86-F4/80</t> and IL-6) and M2 (CD206-F4/80 and IL-10) macrophages markers. Scale bar: 100 µm; (F) MASSON staining of skin tissues on Day 3 and 6. Black square: collagen deposition; black dashed line: the boundary of epidermis and dermis; black circle: hair follicles. Scale bar: 200 µm and 100 µm (magnification).
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    Experimental design for cutaneous wound model and treatment. (A) Scheme of R 2 F 4 R 2 for treating cutaneous wound; (B) Schematic images of wound contraction during 6 d; (C) SEM images of E. coli entrapped by R 2 F 4 R 2 in vivo . Scale bar: 5 µm and 2 µm (magnification); (D) Histopathological H&E staining of skin tissues on Day 3 and 6. The red arrow: epidermis. Blue arrows: ermis; black dashed line: the boundary of epidermis and dermis. Black arrow: the infiltration of inflammatory cells; red circle: hair follicles; black circle: the vasodilation and congestion. Scale bar: 100 µm and 50 µm (magnification); (E) Immunofluorescence analysis of KI67, M1 <t>(CD86-F4/80</t> and IL-6) and M2 (CD206-F4/80 and IL-10) macrophages markers. Scale bar: 100 µm; (F) MASSON staining of skin tissues on Day 3 and 6. Black square: collagen deposition; black dashed line: the boundary of epidermis and dermis; black circle: hair follicles. Scale bar: 200 µm and 100 µm (magnification).
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    Image Search Results


    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Journal: Molecular Therapy Oncology

    Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

    doi: 10.1016/j.omton.2026.201185

    Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

    Techniques: Flow Cytometry

    Experimental design for cutaneous wound model and treatment. (A) Scheme of R 2 F 4 R 2 for treating cutaneous wound; (B) Schematic images of wound contraction during 6 d; (C) SEM images of E. coli entrapped by R 2 F 4 R 2 in vivo . Scale bar: 5 µm and 2 µm (magnification); (D) Histopathological H&E staining of skin tissues on Day 3 and 6. The red arrow: epidermis. Blue arrows: ermis; black dashed line: the boundary of epidermis and dermis. Black arrow: the infiltration of inflammatory cells; red circle: hair follicles; black circle: the vasodilation and congestion. Scale bar: 100 µm and 50 µm (magnification); (E) Immunofluorescence analysis of KI67, M1 (CD86-F4/80 and IL-6) and M2 (CD206-F4/80 and IL-10) macrophages markers. Scale bar: 100 µm; (F) MASSON staining of skin tissues on Day 3 and 6. Black square: collagen deposition; black dashed line: the boundary of epidermis and dermis; black circle: hair follicles. Scale bar: 200 µm and 100 µm (magnification).

    Journal: Asian Journal of Pharmaceutical Sciences

    Article Title: Dual-functional self-assembled nanosystems with enhanced protease resistance: Promoting bacterial aggregation and immune activation for multidrug-resistant bacterial infection

    doi: 10.1016/j.ajps.2026.101145

    Figure Lengend Snippet: Experimental design for cutaneous wound model and treatment. (A) Scheme of R 2 F 4 R 2 for treating cutaneous wound; (B) Schematic images of wound contraction during 6 d; (C) SEM images of E. coli entrapped by R 2 F 4 R 2 in vivo . Scale bar: 5 µm and 2 µm (magnification); (D) Histopathological H&E staining of skin tissues on Day 3 and 6. The red arrow: epidermis. Blue arrows: ermis; black dashed line: the boundary of epidermis and dermis. Black arrow: the infiltration of inflammatory cells; red circle: hair follicles; black circle: the vasodilation and congestion. Scale bar: 100 µm and 50 µm (magnification); (E) Immunofluorescence analysis of KI67, M1 (CD86-F4/80 and IL-6) and M2 (CD206-F4/80 and IL-10) macrophages markers. Scale bar: 100 µm; (F) MASSON staining of skin tissues on Day 3 and 6. Black square: collagen deposition; black dashed line: the boundary of epidermis and dermis; black circle: hair follicles. Scale bar: 200 µm and 100 µm (magnification).

    Article Snippet: Subsequently, cells were exposed to fluorescence-labeled antibodies from Elabscience, specifically F4/80 (E-AB-F0995C), CD206 (E-AB-F1135E) and CD86 (E-AB-F0994D), following the guidelines of the manufacturer.

    Techniques: In Vivo, Staining, Immunofluorescence